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KMID : 0363120230360010060
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Korean Journal of Pain
2023 Volume.36 No. 1 p.60 ~ p.71
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A positive feedback loop of heparanase/syndecan1/nerve growth factor regulates cancer pain progression
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Su Xiaohu
Wang Bingwu
Zhou Zhaoyun
Li Zixian
Tong Song
Chen Simin
Zhang Nan
Liu Su
Zhang Maoyin
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Abstract
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Background: The purpose of this research was to assess the role of heparanase (HPSE)/syndecan1 (SDC1)/nerve growth factor (NGF) on cancer pain from melanoma.
Methods: The influence of HPSE on the biological function of melanoma cells and cancer pain in a mouse model was evaluated. Immunohistochemical staining was used to analyze HPSE and SDC1. HPSE, NGF, and SDC1 were detected using western blot. Inflammatory factors were detected using ELISA assay.
Results: HPSE promoted melanoma cell viability, proliferation, migration, invasion, and tumor growth, as well as cancer pain, while SST0001 treatment reversed the promoting effect of HPSE. HPSE up-regulated NGF, and NGF feedback promoted HPSE. High expression of NGF reversed the inhibitory effect of HPSE down-regulation on melanoma cell phenotype deterioration, including cell viability, proliferation, migration, and invasion. SST0001 down-regulated SDC1 expression. SDC1 reversed the inhibitory effect of SST0001 on cancer pain.
Conclusions: The results showed that HPSE promoted melanoma development and cancer pain by interacting with NGF/SDC1. It provides new insights to better understand the role of HPSE in melanoma and also provides a new direction for cancer pain treatment.
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KEYWORD
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Cancer Pain, Cell Proliferation, Cell Survival, Feedback, Melanoma, Nerve Growth Factor, Pain Management, Syndecan-1
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